Quantitative Real-Time PCR

Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the same in all samples and in the external quantified standards. Consequently, it is important that the efficiency of the PCR does not vary greatly due to minor differences between samples. Careful optimisation of the PCR conditions is therefore required. The use of probes in quantitative realtime PCR improves its performance and a range of suitable systems is now available. Generally quantitative real-time assays have excellent performance characteristics including a wide dynamic range, high sensitivity and accuracy. This has led to their use in a wide range of applications and two examples are presented. Viral quantification is now an important factor in the control of infection. The problems associated with virus quantification in cytomegalovirus (HCMV) infection are similar to those presented by other viruses. Quantitative PCR is finding an increasing role in the diagnosis of cancer. The assessment of c-erbB2/Her2/neu gene duplication is useful in predicting the disease Bacterial Gene Regulation and Transcriptional Networks Edited by: M Madan Babu c. 280 pp, January 2013 ISBN: 978-1-908230-14-0, $319/£159 The latest research observations and current theories on transcriptional regulation and gene circuits in bacteria. Two-Component Systems in Bacteria Edited by: R Gross, D Beier c. 410 pp, August 2012 ISBN: 978-1-908230-08-9, $360/£180 Structure-function analysis, sensing mechanisms, atypical two-component systems, stress responses, developmental processes, virulence and symbiosis. Stress Response in Microbiology Edited by: JM Requena x + 436 pp, June 2012 ISBN: 978-1-908230-04-1, $360/£180 Comprehensively reviews the recent findings that have greatly advanced the understanding of stress response systems. Bacterial Regulatory Networks Edited by: AAM Filloux xiv + 354 pp, June 2012 ISBN: 978-1-908230-03-4, $360/£180 Authoritative, up-to-date reviews of the current research and theories. Critical reviews by leading research scientists. Systems Microbiology: Current Topics and Applications Edited by: BD Robertson, BW Wren xii + 170 pp, June 2012 ISBN: 978-1-908230-02-7, $319/£159 Cutting-edge reviews by worldleading experts. Theoretical approaches, mathematical modelling, case studies on species and the systems analysis of microbial phenomena. Sensory Mechanisms in Bacteria: Molecular Aspects of Signal Recognition Edited by: S Spiro, R Dixon x + 268 pp, September 2010 ISBN: 978-1-904455-69-1, $319/£159 An international team of experts reviews a selection of important model systems, providing a timely snapshot of the current state of research in the field. “an excellent volume” (Microbiol. Today) ORDER FROM (UK/Europe): Caister Academic Press, c/o Book Systems Plus, 1st Floor, 8 Hill St., Saffron Walden, Essex, CB10 1JD, UK Tel: 01799 524458 Fax: 01799 524459 http://uk.caister.com ORDER FROM (USA): Caister Academic Press, c/o ISBS, Inc., 920 NE 58th Avenue, Suite 300, Portland OR 97213-3786, USA Tel: 503 287-3093 Fax: 503 280-8832 http://usa.caister.com FULL DETAILS OF ALL OUR BOOKS AT WWW.CAISTER.COM COMING SOON Regulatory Networks Quantitative Real-time PCR in Applied Microbiology Edited by: Martin Filion x + 242 pp, May 2012 ISBN: 978-1-908230-01-0, $319/£159 Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software. PCR Troubleshooting and Optimization The Essential Guide Edited by: S Kennedy, N Oswald viii + 236 pp, January 2011 ISBN: 978-1-904455-72-1, $319/£159 Control, optimize and troubleshoot PCR, reverse transcriptase PCR, realtime PCR and quantitative PCR. An essential book. “an essential book ... a valuable tool to all those interested in PCR” (Doodys); “an essential guide” Aus. J. Med. Sci. Real-Time PCR in Food Science Current Technology and Applications Edited by: D Rodriguez-Lazaro c. 280 pp, January 2013 ISBN: 978-1-908230-15-7, $319/£159 An indispensable manual on realtime PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens. Real-Time PCR: Advanced Technologies and Applications Edited by: Nick Saunders and Martin A. Lee An essential manual presenting a comprehensive guide to the most upto-date technologies and applications as well as providing vital insights into the theory of this important technique. ... coming so on! PCR Books ... in stock n ow! ... forthcomi ng! ... in stock n ow! • Bionanotechnology: Biological Selfassembly and its Applications • Real-Time PCR in Food Science: Current Technology and Applications • Bioremediation of Mercury: Current Research and Industrial Applications • Neurospora: Genomics and Molecular Biology • Rhabdoviruses: Molecular Taxonomy, Evolution, Genomics, Ecology, HostVector Interactions, Cytopathology and Control • Horizontal Gene Transfer in Microorganisms • Microbial Ecological Theory: Current Perspectives • Malaria Parasites: Comparative Genomics, Evolution and Molecular Biology AVAILABLE NOW • Foodborne and Waterborne Bacterial Pathogens: Epidemiology, Evolution and Molecular Biology • Yersinia: Systems Biology and Control • Bacterial Spores: Current Research and Applications • Small DNA Tumour Viruses “invaluable for its multiple perspectives and concise summary of a large body of research” (MedicalScienceBooks.com) • Extremophiles: Microbiology and Biotechnology “a good overview” (Biospektrum); “recommended” Microbiol. 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